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          1. Health Monitoring Program

            Facility Control and Maintenance

            Mice intended for export are housed in a strictly controlled barrier facility with limited access granted only to trained laboratory and animal care personnel via key card access. Personal protective equipment, including disposable gowns, shoe covers, gloves, hair bonnets, and masks, is required for entry into the facility. Animal care personnel are dedicated to the facility and/or room to ensure strict adherence to facility protocol. Mice are housed in positive-pressure, individually ventilated cages/units (IVC/U) that are changed every 1-2 weeks. Corncob bedding and paper nest enrichment are provided to the mice, and irradiated feed is supplied. Prior to use, all caging, bedding, and enrichment are autoclaved to ensure sterility. An Edstrom automatic watering system supplies processed water (reverse osmosis, chlorinated). Once mice leave the barrier facility, they cannot return.

            Health Status

            The mice housed in the GemPharmatech facility maintain a specified and opportunistic pathogen-free (SOPF) status or germ-free (GF) status. The health status of the animals is meticulously monitored through various methods, including the use of sentinel mice, random sampling within the colony, and exhaust air dusts (EAD) testing. Detailed information regarding these monitoring procedures is described below.

            Sentinel mice: The health status of all colonies is monitored on a quarterly basis using sentinel mice. These sentinel cages contain two female mice aged between 4-6 weeks. Biweekly, the sentinel mice are exposed to soiled bedding collected from cages within their respective racks. The soiled bedding samples are pooled from multiple cages. Each rack unit contains 70 or 80 cages, and the sentinel mice are housed two per cage on each side of the rack unit. The results of these tests are reported as the number of positive samples out of the total number of testing samples. The health monitoring program includes necropsy examinations, detection of parasites, bacteria, and viruses.

            Colony random sample testing: To detect any potential microbiological issues within the colony, live mice, feces, or oral swabs are randomly sampled from immunodeficient mice and large colonies such as C57BL/6, BALB/c ,NCG mice, etc.

            EAD testing: Monthly, exhaust air dust is collected for pathogen detection through EAD testing.

            Importing Animals

            For imported mice, only animals that are specific pathogen-free (SPF) are accepted. However, they must undergo rederivation and testing before being released into the barrier facility. These animals are initially quarantined in isolators, and samples such as hair, feces, or serology samples are collected and submitted to the GemPharmatech microbial testing center for analysis. Only after passing microbial tests can the animals be transported to the breeding rooms via cesarean section or embryo transfer.

            VIRUSES Method
            Mouse Thymic Virus (MTV) PCR
            Ectromelia virus (Ect) PCR
            Mouse hepatitis virus (MHV) ELISA
            Sendai virus (SV) ELISA
            Pneumonia virus of mice (PVM) ELISA
            Reovirus-3 (Reo-3) ELISA/PCR
            Minute virus of mice (MVM) PCR
            Lymphocytic choriomeningitis (LCMV) ELISA/PCR
            Hantavirus (HV) ELISA/PCR
            Theiler's murine encephalomyelitis virus (TMEV) ELISA/PCR
            Mouse Adenovirus FL (MAD1) PCR
            Mouse Adenovirus K87 (MAD 2) PCR
            Polyoma Virus (POLY) ELISA/PCR
            Mouse Parvovirus (MPV) PCR
            Mouse Cytomegalovirus (MCMV) PCR
            Murine Norovirus (MNV) PCR
            Epizootic Diarrhea of Infant Mice (EDIM) PCR
            K virus PCR
            LDH elevating virus (LDV) PCR
            Murine chapparvovirus (MKPV) PCR
            BACTERIA & MYCOPLASMA Method
            Proteus mirabilis Culture
            Pasteurella multocida Culture
            Klebsiella oxytoca Culture
            Filobacterium rodentium (CAR Bacillus) PCR
            Helicobacter spp. (e.g. H. hepaticus, H. bilis, H. muridarum, H. rodentium) PCR
            Pneumocystis spp. PCR
            Citrobacter rodentium Culture
            Bordetella Bronchus Culture
            Mycoplasma spp. PCR
            Salmonella spp. Culture
            Mycoplasma?pulmonis PCR
            Corynebacterium?kutscheri Culture
            Tyzzer's?organism PCR
            Pasteurella?pneumotropica (Rodentibacter pneumotropicus, Rodentibacter heylii) Culture
            Klebsiella pneumoniae Culture
            Staphylococcus aureus Culture
            Pseudomonas aeruginosa Culture
            Pathogenic dermal fungi PCR
            Streptobacillus moniliformis Culture
            Corynebacterium?bovis PCR
            PARASITES & PROTOZOA Method
            Eimeria spp. Micro
            Entamoeba Micro
            Endoparasites (tapeworms, pinworms, and other helminths) Micro
            Flagellatas (e.g. Giardia muris, Spironucleus muris, etc.) Micro
            Toxoplasma gondii PCR
            Ectoparasites, e.g., fleas, mites, and lice Micro
            Ciliata Micro
            Encephalitozoon?cunicul PCR
            OTHER Method
            Pinworms PCR
            Furmites PCR


            Additionally, GemPharmatech partners with several respected institutions and organizations to breed and house mice. For information on the health monitoring and testing practices of these facilities, please contact us.

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